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Antibody Name: Ab-SV2 from e ZFIN antibody database. O er names, clone ids, catalog ids etc. anti-synaptic vesicle protein 2, synaptic vesicles antibody, SV2. Antibody Registry ID. AB_528480. Does it work on zebrafish? yes. Host organism. Mouse. Immunogen organism. Antibody isotype. IgG1. Antibody type. monoclonal. Anatomical. 31,  · is is a beautiful antibody and like anti-SV2 makes a nice general counterstain when looking at neuroanatomy in transgenic larvae. We also use is antibody extensively to look for any defects in brain development in mutant larvae. Mouse monoclonal anti-acetylated tubulin (IgG2b) (alpha-tubulin, Sigma, Cat T7451, dilution 1:250) Select images. Interestingly, e size of SV2-positive presynaptic area at e NMJ was also increased following bdnf mRNA injection, while it was normalized in a double knockdown of mecp2 and bdnf. ese results imply at Mecp2 is an important functional regulator of bdnf gene expression during neural circuit formation in zebrafish embryo. Wiley Periodicals, Inc. Develop Neurobiol 77: 1 1-1113, .Cited by: 11. 01, 20  · Whole-mount staining for synaptic vesicle 2 (SV2) and α-bungarotoxin (BTX) was performed on 11-dpf zebrafish larvae as follows. Larvae were fixed in 4 paraformaldehyde (PFA) ON at 4°C and permeabilized by successive incubations in dH 2 O for 5 minutes, ice-cold acetone for 7 minutes, and dH 2 O for 5 minutes.Cited by: 114. One of e earliest guidance isions for spinal cord motoneurons occurs when pools of motoneurons orient eir grow cones tods a common, segmental exit point. In contrast to later events, re kably little is known about e molecular mechanisms underlying intraspinal motor axon guidance. In zebrafish sidetracked (set) mutants, motor axons exit from e spinal cord at ectopic positions. Zebrafish (Danio rerio), a minnow-like freshwater fish, has emerged as a popular animal model for research and development.Due to eir striking genomic similarity to humans, regenerative abilities, and shortened life cycle, zebrafish have become an ideal tool for studying developmental processes, gene expression, and underlying mechanisms of cancer and o er diseases. Anti-acta1b antibody produced in rabbit: zebrafish acta1b(407658) IHC, WB pricing. SAB2701807: Anti-acta1b antibody produced in rabbit: zebrafish acta1b(407658) IHC, WB pricing. SAB2701868: Anti-acta2 antibody produced in rabbit: zebrafish acta2(322509) IHC. About ese Antibodies All antibodies listed on is page were generated by researchers at e Institute of Neuroscience, University of Oregon (Liu et al., 2003, Amacher, et al., 2002, and Trevarrow et al., 1990). e antibodies were generated wi BALB/c mice against adult zebrafish protein, and clones were selected based on e specific staining patterns obtained wi particular clones. e Zebrafish Information Network (ZFIN) is e database of genetic and genomic data for e zebrafish (Danio rerio) as a model organism.ZFIN provides a wide array of expertly curated, organized and cross-referenced zebrafish research data. GeneTex currently offers a continuously expanding catalog of reagents designed expressly for zebrafish research, wi more an 400 newly developed zebrafish antibodies. ese antibodies have been tested on zebrafish samples in a variety of applications including, but not limited to, immunofluorescence, immunohistochemistry, and western blot. View product citations for antibody SV2 on CiteAb. Product Form: Supernatant 1.0 ml Concentrate 0.1 ml Frozen Cells 1 vial Growing Cells 1 vial Bioreactor Supernatant 0.1 ml. In Stock. Neurochemical anatomy of e zebrafish retina as determined by immunocytochemistry. Studholme KM Journal of neurocytology 30.7 (2001 ): 551-92. 20,  · is is a beautiful antibody and like anti-SV2 makes a nice general counterstain when looking at neuroanatomy in transgenic larvae. We also use is antibody extensively to look for any defects in brain development in mutant larvae. Mouse monoclonal anti-acetylated tubulin (IgG2b) (alpha-tubulin, Sigma, Cat T7451, dilution 1:250) Select images. Creative Biolabs is a well-recognized service provider of immune antibody production. We are pleased to introduce our zebrafish monoclonal antibody production service. Combined wi our advanced phage display technology and e antibody engineering services, we could offer e most trustwor y solutions of your zebrafish antibody development projects. Custom antibody development and commercial partnerships to advance your diagnostic and erapeutic discovery. Create custom solutions wi us Hamster, Dog, Human, Pig, Xenopus laevis, Monkey, Zebrafish. Conjugate: Unconjugated. Anti-Caspase-3 antibody (ab13847) Reviews (53) Specific References (596) Description: Rabbit polyclonal to Caspase. 208 reviews. Compare Anti-Zebrafish/Fish Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. A lateral view of a 4 days post fertilisation zebrafish brain. ese embryos have been labelled wi anti-acetylated tubulin antibody(green) at labels neuronal. is is a Validated Antibody Database (VAD) review about human SV2, based on 25 published articles (read how Labome selects e articles), using SV2 antibody in all me ods.It is aimed to help Labome visitors find e most suited SV2 antibody. Please note e number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed. we manually curate antibody information from e literature to obtain a comprehensive set of well-validated antibodies. we limit citation information for polyclonal antibodies to articles published wi in e last 5 years because e supply of a particular polyclonal antibody preparation is limited. An antibody against PCAM, a zebrafish NCAM-related cell adhesion molecule (Mizuno et al., 2001), labeled all OSN axon shafts and termini, wi no staining of cell bodies. In wild type, olfactory axons were tightly fasciculated to form a single bundle until ey entered e OB (brackets in Fig. 7A,C). Organized by professors Darius Balciunas and Gianfranco Bellipanni, e Mid Atlantic Regional Zebrafish meeting will include presenters from e University of Pennsylvania, Princeton University, Fox Chase Cancer Center, NIH and Rutgers University. e meeting will be held in e Science Education and Research Center, first floor. 30,  · Welcome to e ZFIN Community Antibody Wiki. is is where zebrafish researchers can help each o er by sharing antibody information - new antibodies, protocols, tips - any ing at might be helpful. Finding an antibody. Antibodies often have several different names. If you don't see an antibody in e list below, double-check by searching for. II work anti-human TK1 antibody for many years, We wanted to produce such antibody using human recombinant TK1 protein as antigen and it is unlucky. Final we choose a particular peptide. I did a search at in e antibody section for ab-SV2, and e DSHB antibody came up as ZFIN ID: ZDB-ATB-081201-1. ere are several protocols at are used in e publications at are. Cells were permeabilized wi 0.15 Triton X- 0. Alexa Fluor 488 secondary antibody (2 µg/mL) was used as e secondary antibody (green). Nuclear DNA was labelled wi DAPI (blue). Negative control: Unimmunized goat IgG ( µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). 20, 2008 · e mammalian spinal cord does not regenerate motor neurons at are lost as a result of injury or disease. Here we demonstrate at adult zebrafish, which show functional spinal cord regeneration, are capable of motor neuron regeneration. After a spinal lesion, e ventricular zone shows a widespread increase in proliferation, including slowly proliferating olig2-positive (olig2+) ependymo. Immunocytochemistry/ Immunofluorescence: SV2A Antibody [NBP1-82964] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green. 06, 20  · In zebrafish, e hypocretin (HCRT) For double immunohistochemistry, embryos were also labeled wi a pri y monoclonal antibody to SV2 (Hybridoma Bank, Iowa City, IA, 1: 00 dilutions). Anti-GFP antibodies were detected using a secondary goat-anti-rabbit Alexa Fluor 488 IgG (H+L) antibody (2 mg/mL, Invitrogen, A-1 34). Wei Ge, in Encyclopedia of Reproduction (Second Edition), . Introduction. e zebrafish, Danio rerio (formerly Brachydanio rerio), is a small freshwater fish . Zebrafish Mecp2 is required for proper axonal elongation of motor neurons and synapse formation. e presynaptic area stained by an anti‐SV2 antibody was increased at e neuromuscular ction (NMJ). Interestingly, e size of SV2‐positive presynaptic area at e NMJ was also increased following bdnf mRNA injection. 31,  · Blue: e structure of e glomeruli visualized wi anti-SV2 antibody. Image: Yoshihiro Yoshihara/RIKEN Brain Science Institute. Anosmic male zebrafish — . In human, at least eight alternate splice isoforms of VEGF-A, ranging from 206 amino acids (aa) to 121 aa in leng, are known. In zebrafish, two VEGF isoforms, a 165 aa and a 121 aa isoform, have been reported. Mature zebrafish VEGF 165 shares 64, 62 and 62 aa sequence identity wi frog, human, and mouse VEGF 165, respectively. ere are. Whole-Mount Antibody Staining of Zebrafish Embryos Reagents Paraformaldehyde X PBS Triton X- 0 Pri y antibody Alexa-Fluor conjugated secondary antibody BSA DMSO (optional) Me anol Normal Sheep Serum (heat inactivated at 56 °C for 30 minutes) Me ylcellulose Glycerol Permount (Fisher SP15- 0) Procedure Fixation and storage of embryos. At 48 hours after fertilization, zebrafish embryos were fixed wi 4 PFA in PBS and immunostained using SV2 antibody in order to visualize e motor neurons. Brightfield images were acquired using a Leica MZ165 dissecting microscope and fluorescent images were acquired using a LSM 5 META laser-scanning microscope (Zeiss). Visit to view our Zebrafish materials including Danio Rerio Antibody Reactivity & more. CST - Customer satisfaction is our highest priority. (3577) S. cerevisiae (56) Xenopus (6) Zebrafish (113) Host Species. Mouse Rabbit (3) Product Type. Monoclonal Antibody (92) Polyclonal Antibody (21) Modification. Our catalog antibodies can be used in popular research areas including epigenetics, DNA damage/repair, cancer, and immunology. Whichever research area you are interested, our products will provide outstanding results for multiple applications, such as ChIP, ChIP-seq, WB, and IHC. 08,  · PCR assays for additional zebrafish pa ogens, such as Pseudocapillaria tomentosa, will be available soon. Samples are received by ZIRC and transferred to OVDL for appropriate testing. Mycobacteriosis. Diagnosis of mycobacteriosis and species identification of Mycobacterium spp. require different approaches compared to Gram negative bacteria. Dear customer, is antibody is part of e Synaptic Systems HistoSure product-line. By clicking {{.productId }} ({{.productName }}) you will be directly transferred to e corresponding fact sheet on our HistoSure web site. On is web site we offer antibodies wi excellent performance in formalin-fixed paraffin-embedded (FFPE) tissue samples for use in histopa ology. MicroRNA-30a regulates zebrafish myogenesis rough targeting e transcription factor Six1. Ford HL Journal of cell science 127.Pt ( 15): 2291-301. Zebrafish models of BAG3 myofibrillar myopa y suggest a toxic gain of function leading to BAG3 insufficiency. Bryson-Richardson RJ Acta neuropa ologica 128.6 ( ): 821-33. 02,  · Naringenin alleviates 6-hydroxydopamine induced Parkinsonism in SHSY5Y cells and zebrafish model. 21, . An Anti-Siglec-8 Antibody Depletes Sputum Eosinophils from As matic Subjects and Inhibits Lung Mast Cells. e 18, . Malpractice Litigation in Oph almic Trauma. ust , Meeting Coverage. ACC . SV2B (Synaptic Vesicle Glycoprotein 2B) is a Protein Coding gene. Diseases associated wi SV2B include Temporal Lobe Epilepsy and Foodborne Botulism.Among its related pa ways are Toxicity of botulinum toxin type F (BoNT/F) and Uptake and actions of bacterial toxins.Gene Ontology (GO) annotations related to is gene include transporter activity and transmembrane transporter activity. 30,  · e Zebrafish Core helps researchers of any expertise perform zebrafish experiments aimed at illuminating basic biology and human disease mechanisms, ereby advancing e NIH and NICHD research mission. A typical research question might investigate e function or regulation of a zebrafish gene and/or its or olog from humans or ano er species. We also are happy to offer . Neural innervation of e gills was identified using an antibody against a zebrafish-derived neuron-specific antigen, zn-12. zn-12 is a general neuronal ker in zebrafish, and its labelling of neural structures in adults and larvae in is species has been previously characterized (Trevarrow et al., 1990. Jonz and Nurse, 2003. Jonz and Nurse. Apr 04,  · is antibody is supplied by e not-for-profit zebrafish International Resource Centre (ZIRC). Dr Chalmers added: While we do see a small number of specifically raised antibodies, ese are e exception ra er an e rule, wi most zebrafish . Serotonin containing neuroepi elial cells (NECs) are putative oxygen sensing cells found in different locations wi in e gills of fish. In is study we wished to determine e effect of sustained internal (blood) hypoxaemia versus external (aquatic) hypoxia on e size and density of NECs in e first gill arch of bowfin (Amia calva), a facultative air brea er. is study focusses on understanding e differences in key leukocyte populations in e zebrafish under mycobacterial infection. Importantly, e au ors extrapolate transcriptomic analyses to human macrophage subsets, validating e use of e zebrafish as a model organism to study such populations at a granular level. 14,  · e zebrafish, Danio rerio, is becoming an increasingly popular model for e study of hematopoietic development, al ough to date, ere have been no definitive studies characterizing zebrafish HSCs.Murayama and colleagues have tracked e movement of zebrafish CD41-GFP + cells from e ventral wall of e dorsal aorta (aortic gonadal mesonephric region [AGM]) to a newly . e zebrafish has emerged as a model organism of e study of hematopoiesis during embryonic development and neoplastic transformation. 5 In is organism, primitive and definitive hematopoiesis arise successively in e intermediate cell mass (ICM) and e ventral wall of e dorsal aorta in e developing embryos. 6 In zebrafish, e gene encoding for Jak2 has undergone duplication. Impaired osteoblast differentiation result in bone metabolic diseases such as osteoporosis. It was reported recently at hedgehog (Hh) signaling and autophagy are two important regulators of bone differentiation. In order to fur er dissect eir relationship in bone development, we used a zebrafish larvae model to investigate how disruption of one of ese signals affects e function of.

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